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一氧化氮可增加微生物耐輻射性

時間:2009-10-26 05:43|來源:生物谷| | 分享|點擊:


一氧化氮很可能是耐輻射奇球菌(Deinococcus Radiodurans)具有耐受能殺死更大生物的輻射劑量的能力的關鍵原因。這種堅韌的原核生物被用于修復放射性廢料地點,而一些耐輻射奇球菌在罐頭食品產品中被發現。這種生物被稱為“世界上最堅韌的”細菌,這是由于它能耐受極端溫度、嚴重脫水以及致命劑量的輻射。 織夢好,好織夢

Brian Crane及其同事研究了這種細菌的輻射生存能力,結果發現了接觸紫外線能增強它的一氧化氮合酶的產量,這種酶負責制造一氧化氮。在動物中,一氧化氮被證明在一系列的生物過程中扮演了一個角色,包括防止病原體,調控血管張力、激素釋放以及神經元信號傳導。這組作者使用了一種遺傳改造過的耐輻射奇球菌,它的一氧化氮合酶被去除了。結果他們發現,盡管這種生物能耐受其他應激物(包括接觸自由基),它們在接觸輻射之后的生存不良。(生物谷Bioon.com) 織夢好,好織夢

原始出處: 內容來自dedecms

PNAS October 19, 2009, doi: 10.1073/pnas.0907262106 copyright dedecms

Endogenous nitric oxide regulates the recovery of the radiation-resistant bacterium Deinococcus radiodurans from exposure to UV light 本文來自織夢

Bhumit A. Patela, Magali Moreaua, Joanne Widoma, Huan Chenb, Longfei Yinb, Yuejin Huab and Brian R. Cranea,1

本文來自織夢

aDepartment of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853; and
bInstitute of Nuclear-Agricultural Sciences, Zhejiang University, Hangzhou 310008, China 織夢好,好織夢

Deinococcus radiodurans (Dr) withstands desiccation, reactive oxygen species, and doses of radiation that would be lethal to most organisms. Deletion of a gene encoding a homolog of mammalian nitric oxide synthase (NOS) severely compromises the recovery of Dr from ultraviolet (UV) radiation damage. The Δnos defect can be complemented with recombinant NOS, rescued by exogenous nitric oxide (NO) and mimicked in the wild-type strain with an NO scavenging compound. UV radiation induces both upregulation of the nos gene and cellular NO production on similar time scales. Growth recovery does not depend on NO being present during UV irradiation, but rather can be manifested by NO addition hours after exposure. Surprisingly, nos deletion does not increase sensitivity to oxidative damage, and hydrogen peroxide does not induce nos expression. However, NOS-derived NO upregulates transcription of obgE, a gene involved in bacterial growth proliferation and stress response. Overexpression of the ObgE GTPase in the Δnos background substantially alleviates the growth defect after radiation damage. Thus, NO acts as a signal for the transcriptional regulation of growth in D. radiodurans. 本文來自織夢

Tags:輻射,增加,of,the,and,NO,生物,nos,這種,
責任編輯:實驗室前沿

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